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Render Timestamp: 2024-11-21T13:08:02.168Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:59:08.128
Product last modified at: 2024-10-30T12:45:45.431Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Transferrin (E7F4T) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Transferrin (E7F4T) Rabbit mAb #35293

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Transferrin (E7F4T) Rabbit mAb recognizes endogenous levels of total transferrin protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg663 of human transferrin protein.

    Background

    Transferrin is the major ferric iron transport protein, which binds ferric (Fe3+) ions in association with the binding of an anion, usually bicarbonate (1). The iron binding affinity of transferrin is pH dependent. In a neutral pH environment, transferrin (apotransferrin) binds iron with high affinity to form iron-bound transferrin (holotransferrin). In an acidic pH environment, the affinity of iron bound to transferrin decreases, dissociating iron from holotransferrin and releasing it into the environment (2). The importance of holotransferrin is to sequester Fe3+ ions in a relatively nonreactive and inert state to ensure normal free iron homeostasis in the body (3). Transferrin has been shown to contribute to iron-related oxidative damage (4), DNA synthesis (5), cell growth (6), and differentiation (7). Holotransferrin delivers iron to cells by binding to transferrin receptor (TFR1 or TFR2). Neutral pH at the cell surface promotes binding of holotransferrin to TFR1/TFR2. The receptor-ligand complex enters the cell through receptor-mediated endocytosis and is internalized into an endosome. Relatively lower endosomal pH results in the release of iron. The receptor-ligand complex is recycled to the cell surface, where apotransferrin dissociates from TFR1/TFR2 (8).

    Database Links

    UniProt ID: P02787


    Entrez-Gene Id: 7018


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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