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Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:16:49.445
Product last modified at: 2025-02-27T12:15:26.688Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Transgelin/TAGLN (E6Y8S) Rabbit mAb #52011

Filter:
  • WB
Western Blotting Image 1: Transgelin/TAGLN (E6Y8S) Rabbit mAb
Western blot analysis of extracts from various cell lines using Transgelin/TAGLN (E6Y8S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of Transgelin/TAGLN protein in Colo 205 cells is consistent with the predicted expression pattern.

To Purchase # 52011

Cat. # Size Qty. Price
52011T 20 µl
$153
52011S 100 µl
$339

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 23
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Transgelin/TAGLN (E6Y8S) Rabbit mAb recognizes endogenous levels of total Transgelin/TAGLN protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly51 of human Transgelin/TAGLN protein.

Background

Transgelin (TAGLN, SM22α) is an actin-binding protein of the calponin family. It contains an amino-terminal calponin homology domain and a carboxy-terminal calponin-like module domain required for actin binding (1,2). TAGLN is a TGF-β inducible gene that is abundantly expressed in visceral and vascular smooth muscle cells, and is often used as an early marker of smooth muscle differentiation (3-5). TAGLN expression has also been shown in brain pericytes, providing use in studying the blood brain barrier (6). Altered levels of TAGLN are seen in multiple cancer types, including colorectal, breast, and prostate cancer (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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