Render Target: STATIC
Render Timestamp: 2024-11-25T11:33:11.281Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-11-07 16:01:06.627
Product last modified at: 2024-11-19T22:15:14.877Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Transgelin/TAGLN (F3B7H) Rabbit mAb #36090

Filter:
  • WB
  • IHC
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 23
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunohistochemistry (Paraffin) 1:200 - 1:800
    Immunofluorescence (Frozen) 1:800 - 1:1600
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Transgelin/TAGLN (F3B7H) Rabbit mAb recognizes endogenous levels of total Transgelin/TAGLN protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Transgelin/TAGLN protein.

    Background

    Transgelin (TAGLN, SM22α) is an actin-binding protein of the calponin family. It contains an amino-terminal calponin homology domain and a carboxy-terminal calponin-like module domain required for actin binding (1,2). TAGLN is a TGF-β inducible gene that is abundantly expressed in visceral and vascular smooth muscle cells, and is often used as an early marker of smooth muscle differentiation (3-5). TAGLN expression has also been shown in brain pericytes, providing use in studying the blood brain barrier (6). Altered levels of TAGLN are seen in multiple cancer types, including colorectal, breast, and prostate cancer (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.