Render Target: STATIC
Render Timestamp: 2024-10-30T09:53:14.715Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-04-05 20:45:43.928
Product last modified at: 2024-10-08T21:15:08.991Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

TREX1 Antibody #76892

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 33
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TREX1 Antibody recognizes endogenous levels of total TREX1 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val94 of mouse TREX1 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    TREX1 is a broadly expressed 3’ to 5’ exonuclease that acts on single-stranded DNA (ssDNA) to negatively regulate the interferon-stimulatory DNA (ISD) response (1-4). In humans, there are three TREX1 isoforms generated through alternative splicing with predicted molecular weights of 32, 33, and 39 kDa (2). The transcript for the 33 kDa isoform is the most abundant (2). Mice deficient in TREX1 accumulate intracellular ssDNA, which triggers the ISD response and eventually lethal autoimmunity (3,4). Mutations in TREX1 are associated with autoimmune diseases including Aicardi-Goutieres syndrome and systemic lupus erythematosus (5,6). In addition, TREX1 prevents the cell-intrinsic innate immune response to human immunodeficiency virus (HIV) by digesting excess HIV DNA that would normally trigger induction of type I interferon (7).
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