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Render Timestamp: 2024-12-20T11:10:40.819Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:11.232
Product last modified at: 2024-09-30T08:01:52.774Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. TRIM9 (E5P3R) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TRIM9 (E5P3R) Rabbit mAb #32644

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 70, 79
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TRIM9 (E5P3R) Rabbit mAb recognizes endogenous levels of total TRIM9 protein. This antibody is predicted to cross-react with all three TRIM9 isoforms.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val217 of human TRIM9 protein.

    Background

    Tripartite motif-containing protein 9 (TRIM9) is a brain-enriched E3 ubiquitin-protein ligase. TRIM family proteins contain a zinc-binding domain, followed by RING finger, B-box, and coiled-coil domains (1). TRIM proteins are implicated in several biological processes, including cell development and growth, but their molecular mechanism of action remains unclear. Interestingly, TRIM9 is reported to function as an E3 ubiquitin ligase (2). Ubiquitination is the post-translational modification of protein substrates by ubiquitin, a 76-amino acid polypeptide. This process is important for cellular protein turnover, and alterations in this process are linked to human diseases, including neurodegenerative diseases (3). Protein ubiquitination is mediated by a trio of ubiquitination enzymes, including E3 ubiquitin ligases. As a brain-enriched E3 ubiquitin ligase, TRIM9 may play a role in maintaining proper cellular proteostasis in the brain (2). Alterations in TRIM9 expression may contribute to human neurodegenerative diseases, including Parkinson's disease, or injury (2,4).

    Database Links

    UniProt ID: Q9C026


    Entrez-Gene Id: 114088


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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