TTK (D15B7) Rabbit mAb #5469
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 95 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
TTK also participates in the DNA damage response by directly phosphorylating and activating the cell cycle checkpoint kinase Chk2 at Thr68. Two targets phosphorylated by Chk2 are the cell cycle phosphatase cdc25 and the transcription factor p53. Inactivation of cdc25 phosphatase results in the accumulation of inactive cyclin B and cell cycle arrest following DNA damage. Phosphorylation of p53 by active Chk2 stabilizes the transcription factor and promotes cell cycle arrest and apoptosis in response to DNA damage (10).
- Mills, G.B. et al. (1992) J. Biol. Chem. 267, 16000-16006.
- Stucke, V.M. et al. (2002) EMBO J. 21, 1723-1732.
- Lindberg, R.A. et al. (1993) Oncogene 8, 351-359.
- Fisk, H.A. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 14875-14880.
- Dou, Z. et al. (2003) Cell Res. 13, 443-449.
- Abrieu, A. et al. (2001) Cell 106, 83-93.
- Leng, M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 11485-11490.
- Schmidt, M. et al. (2005) EMBO Rep. 6, 866-872.
- Dorer, R.K. et al. (2005) Curr. Biol. 15, 1070-1076.
- Wei, J.H. et al. (2005) J. Biol. Chem. 280, 7748-7757.
Limited Uses
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