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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Type I Interferon Induction and Signaling Antibody Sampler Kit

Type I Interferon Induction and Signaling Antibody Sampler Kit #43573

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Type I Interferon Induction and Signaling Antibody Sampler Kit provides an economical means of detecting the activation of pathways leading to upregulation of type I interferon (IFN), expression of IFN-β1, activation of signaling downstream of type I IFN, and expression of the MX1 interferon response gene, using phospho-specific and control antibodies. The kit includes enough antibodies to perform at least two western blot experiments.

    Specificity / Sensitivity

    Each antibody in the Type I Interferon Induction and Signaling Antibody Sampler Kit recognizes endogenous levels of its target protein. Phospho-IRF-3 (Ser386) (E7J8G) XP® Rabbit mAb recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser386. Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb recognizes endogenous levels of IRF-7 protein only when phosphorylated at Ser477 and can also detect IRF-7 when dually phosphorylated at Ser477 and Ser479. Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb may cross-react with unidentified proteins of 100 and 150 kDa. Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb recognizes endogenous levels of Stat1 protein only when phosphorylated at Ser727. Stat1 (D1K9Y) Rabbit mAb cross-reacts with an unidentified protein of 150 kDa. IRF-9 (D2T8M) Rabbit mAb cross-reacts with an unidentified protein of 95 kDa.

    Source / Purification

    Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Pro115 of human IRF-7, Pro688 of human Stat1, Leu292 of human MX1, recombinant human IRF-3 protein, recombinant human IFN-β1 protein, and recombinant human IRF-9 protein. Phosphorylation-specific monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Ser386 of human IRF-3, Ser477 of human IRF-7, and Ser727 of human Stat1.

    Background

    The innate immune system uses pattern recognition receptors (PRRs) that detect conserved pathogen-associated molecular patterns (PAMPs), such as cytoplasmic double-stranded RNA, to detect and initiate an immune response to viral infection. Detection of virus by PRRs leads to phosphorylation and nuclear translocation of IRF-3 and IRF-7, resulting in upregulation of type I interferons, which include IFN-α and IFN-β (1-3). Type I interferons signal through the interferon α/β receptor (IFNAR), leading to phosphorylation and activation of Stat1 and Stat2, which form a complex with IRF-9 (4,5). This complex translocates to the nucleus where it induces transcription of interferon response genes including viral restriction factors, such as MX1, that limit viral replication and propagation (4-7).

    Database Links

    UniProt ID: Q92985 , P01574 , Q00978 , P20591 , Q14653 , P42224


    Entrez-Gene Id: 3665 , 3456 , 10379 , 4599 , 3661 , 6772


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
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