UAP56 (E7W7M) Rabbit mAb #47258
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 49 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
UAP56 (E7W7M) Rabbit mAb recognizes endogenous levels of total UAP56 protein. This antibody does not cross-react with DDX39A protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly24 of human UAP56 protein.
Background
The UAP56 gene is found in the central MHC region and encodes a member of the DEAD-box family of RNA helicases (1). Also known as DDX39B and BAT1, UAP56 functions as an ATP-dependent splicing factor and RNA helicase in the evolutionary conserved transcription/export (TREX) complex. The TREX complex is recruited to sites of active transcription, where it travels along the length of the gene with RNA polymerase II and exports resulting mRNAs to the cytoplasm (2-8). Both UAP56 and its paralog DDX39A are hijacked by various viral replication machineries to enable viral reproduction and mRNA export (9-11). UAP56 and DDX39A have also been implicated in promoting the AR-V7 splice variant in advanced prostate cancers (12).
- Peelman, L.J. et al. (1995) Genomics 26, 210-8.
- Fleckner, J. et al. (1997) Genes Dev 11, 1864-72.
- Strässer, K. et al. (2002) Nature 417, 304-8.
- Custódio, N. et al. (2004) RNA 10, 622-33.
- Kapadia, F. et al. (2006) Gene 384, 37-44.
- Shen, J. et al. (2007) J Biol Chem 282, 22544-50.
- Kota, K.P. et al. (2008) J Cell Sci 121, 1526-37.
- Majerciak, V. et al. (2010) Virology 407, 206-12.
- Dufu, K. et al. (2010) Genes Dev 24, 2043-53.
- Zielke, B. et al. (2011) J Virol 85, 1804-19.
- Wisskirchen, C. et al. (2011) J Virol 85, 8646-55.
- Nakata, D. et al. (2017) Biochem Biophys Res Commun 483, 271-276.
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