Render Target: STATIC
Render Timestamp: 2024-11-15T11:00:57.199Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:57:56.496
Product last modified at: 2024-10-28T11:45:47.246Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

UBAP2L (E5X4E) Rabbit mAb #40199

Filter:
  • WB
  • IP
  • IHC
  • IF
  • eCLIP

    Supporting Data

    REACTIVITY H M Mk
    SENSITIVITY Endogenous
    MW (kDa) 150, 160
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • eCLIP-eCLIP 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200
    eCLIP 1:200
    For more information about the RBP-eCLIP service please visit Eclipsebio.

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    UBAP2L (E5X4E) Rabbit mAb recognizes endogenous levels of total UBAP2L protein. This antibody may detect non-specific bands of unknown origin between 80-110 kDa.

    Species Reactivity:

    Human, Mouse, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human UBAP2L protein.

    Background

    Ubiquitin-associated protein 2-like (UBAP2L) is a ubiquitous and highly conserved protein containing an N-terminal ubiquitin-associated (UBA) domain involved in the ubiquitin-proteasome system (UPS) and aggregate formation induced by proteasome inhibitors (1). It can also interact with the Polycomb group protein BMI1 to form a Polycomb subcomplex and regulate hematopoietic stem cell activity (2). UBAP2L is essential for the formation of stress granules (SGs), small cytosolic membraneless organelles that form in eukaryotic cells after stress exposure (3). Some studies suggest that UBAP2L is methylated by the arginine methyltransferase PRMT1, and that this methylation inhibits UBAP2L interaction with SG elements and overall SG assembly (4,5). UBAP2L is also an oncogenic protein found to be amplified and/or overexpressed in a large subset of cancers, including breast cancer, hepatocellular carcinoma, lung adenocarcinoma, glioblastoma, prostate cancer, and colorectal cancer, where it is often associated with tumor growth and poor prognosis (6-12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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