UBE2L3 (D5G1) Rabbit mAb #8721
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 18 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
UBE2L3, also commonly referred to as UBCH7, is a ubiquitin-conjugating enzyme that belongs to a family of four related human genes, UBE2L1-UBE2L4. It appears as though UBE2L3 is the only member of this gene family to be transcribed and translated and that UBE2L1, UBE2L2, and UBE2L4 exist as pseudogenes (2,3). As a ubiquitin-conjugating enzyme, UBE2L3 has been linked to the ubiquitination of numerous substrates via its interaction with protein-ubiquitin E3 ligases such as NEDD4 (4), E6AP (5), Parkin (6), c-Cbl (7), and Triad1 (8,9). There is also evidence suggesting that UBE2L3 can modulate the transcriptional activity of numerous members of the nuclear hormone receptor superfamily such as the glucocorticoid receptor, progesterone receptor, androgen receptor, and retinoic acid receptors (10). Furthermore, UBE2L3 protein levels appear to be regulated by the ubiquitin-proteasome pathway allowing for it to exert control over S-phase entry and progression (11).
- Hershko, A. (1988) J Biol Chem 263, 15237-40.
- Ardley, H.C. et al. (2000) Biochim Biophys Acta 1491, 57-64.
- Moynihan, T.P. et al. (1996) Mamm Genome 7, 520-5.
- Anan, T. et al. (1998) Genes Cells 3, 751-63.
- Huang, L. et al. (1999) Science 286, 1321-6.
- Shimura, H. et al. (2001) Science 293, 263-9.
- Yokouchi, M. et al. (1999) J Biol Chem 274, 31707-12.
- Marteijn, J.A. et al. (2009) Leukemia 23, 1480-9.
- Marteijn, J.A. et al. (2005) Blood 106, 4114-23.
- Verma, S. et al. (2004) Mol Cell Biol 24, 8716-26.
- Whitcomb, E.A. et al. (2009) Mol Biol Cell 20, 1-9.
Limited Uses
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