UBE2O Antibody #83393
- WB
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 200 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val576 of human UBE2O protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).
UBE2O (E2-230K) is a unique member of the E2 family of ubiquitin-conjugating enzymes in that it functions as an E2-E3 hybrid enzyme (2). Research studies have demonstrated that UBE2O expression is regulated during erythroid differentiation, which suggests that its enzymatic activity participates in shaping the architecture of the erythrocyte proteome (3). UBE2O has also been implicated in BMP-7-induced adipocyte differentiation through its regulation of SMAD6 monoubiquitination (4).
Limited Uses
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