UBE2S (D5H9H) Rabbit mAb #11878
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 26 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
The human anaphase promoting complex (APC/C) is a large macromolecular E3 ligase complex that is largely responsible for timely progression through mitosis via the sequential targeting of cell cycle regulators for proteasomal degradation. Recent work has revealed that APC/C substrates are marked for proteasomal degradation during cell cycle progression through the covalent assembly of Lys11-linked ubiquitin chains, which occurs through a priming phase and an elongation phase (2-5). The APC/C utilizes, in part, the UBE2C/UBCH10 E2 enzyme to prime substrates for degradation through the covalent attachment of short Lys11-linked chains (3,6). The Lys11-specific elongating E2 enzyme, UBE2S/E2-EPF, extends these short chains into long Lys11-linked ubiquitin chains on APC/C bound substrates (2,3,7). In addition to the well-established biochemical role for UBE2S in cell cycle regulation, researchers have found evidence that this enzyme is overexpressed in many types of human cancer (8), and has been implicated in hypoxia signaling (9,10). Indeed, UBE2S has been reported by researchers to associate with VHL and to target it for proteasomal degradation, thereby stabilizing HIF-1α (9).
- Hershko, A. (1988) J Biol Chem 263, 15237-40.
- Williamson, A. et al. (2009) Proc Natl Acad Sci U S A 106, 18213-8.
- Jin, L. et al. (2008) Cell 133, 653-65.
- Wu, T. et al. (2010) Proc Natl Acad Sci U S A 107, 1355-60.
- Song, L. and Rape, M. (2010) Mol Cell 38, 369-82.
- Summers, M.K. et al. (2008) Mol Cell 31, 544-56.
- Wickliffe, K.E. et al. (2011) Cell 144, 769-81.
- Tedesco, D. et al. (2007) Neoplasia 9, 601-13.
- Jung, C.R. et al. (2006) Nat Med 12, 809-16.
- Roos, F.C. et al. (2011) Am J Pathol 178, 853-60.
Limited Uses
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