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XML generation date: 2025-04-01 17:33:13.580
Product last modified at: 2025-02-24T08:15:09.022Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Ubiquitin (FK2) Mouse mAb #39269

Filter:
  • WB
  • IP
Western Blotting Image 1: Ubiquitin (FK2) Mouse mAb
Western blot analysis of extracts from HeLa, Daudi, and EL4 cells, untreated (-) or treated with MG-132 (10 µM, 18 hr; +), using Ubiquitin (FK2) Mouse mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174. Increased signal with MG-132 treatment is consistent with the predicted expression pattern.

To Purchase # 39269

Cat. # Size Qty. Price
39269S 100 µg
$217

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG1 kappa
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in PBS (pH 7.2) containing 0.09% sodium azide. Stable for 36 months when stored at 4°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Ubiquitin (FK2) Mouse mAb recognizes endogenous levels of total ubiquitin protein. This antibody detects conjugates but not free ubiquitin.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a crude preparation of polyubiquitinated lysozyme.

Background

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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