Ubiquityl-Histone H2A (Lys119) (D27C4) XP® Rabbit mAb #8240
- WB
- IP
- IF
- F
- ChIP
- C&R
- C&T
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 23 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
- C&T-CUT & Tag
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
| Application | Dilution |
|---|---|
| Western Blotting | 1:2000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:3200 |
| Chromatin IP | 1:100 |
| Chromatin IP-seq | 1:100 |
| CUT&RUN | 1:50 |
| CUT&Tag | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #45772.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
- Liu, F. and Walters, K.J. (2010) Trends Biochem Sci 35, 352-60.
- Wang, H. et al. (2004) Nature 431, 873-8.
- Cao, R. et al. (2005) Mol Cell 20, 845-54.
- de Napoles, M. et al. (2004) Dev Cell 7, 663-76.
- Fang, J. et al. (2004) J Biol Chem 279, 52812-5.
- Ginjala, V. et al. (2011) Mol Cell Biol 31, 1972-82.
- Bergink, S. et al. (2006) Genes Dev 20, 1343-52.
- Marteijn, J.A. et al. (2009) J Cell Biol 186, 835-47.
- Wu, J. et al. (2009) Mol Cell Biol 29, 849-60.
Limited Uses
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