Ubiquityl-Histone H2B (Lys120) (D11) XP® Rabbit mAb #5546
- WB
- IF
- F
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 23 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 2.5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
| Chromatin IP | 1:200 |
| Chromatin IP-seq | 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #33291.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51.
- Liu, F. and Walters, K.J. (2010) Trends Biochem Sci 35, 352-60.
- Kim, J. et al. (2009) Cell 137, 459-71.
- Wood, A. et al. (2003) J Biol Chem 278, 34739-42.
- Xiao, T. et al. (2005) Mol Cell Biol 25, 637-51.
- Minsky, N. et al. (2008) Nat Cell Biol 10, 483-8.
- Pavri, R. et al. (2006) Cell 125, 703-17.
- Fleming, A.B. et al. (2008) Mol Cell 31, 57-66.
- Shilatifard, A. (2006) Annu Rev Biochem 75, 243-69.
- Wyce, A. et al. (2004) Novartis Found Symp 259, 63-73; discussion 73-7, 163-9.
Limited Uses
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