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XML generation date: 2024-11-19 18:01:09.789
Product last modified at: 2024-12-17T13:00:12.282Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. UFMylation Antibody Sampler Kit

UFMylation Antibody Sampler Kit #63522

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The UFMylation Antibody Sampler Kit provides an economical means of detecting proteins associated with UFMylation during ER-phagy. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Background

    The ubiquitin-fold modifier 1 (UFM1) conjugation system (UFMylation) is a ubiquitin-like system that plays key roles in development and stress responses (1, reviewed in 2-4). Like ubiquitin, conjugation of UFM1 is a three-step enzymatic process involving a specific E1-activating enzyme (UBA5), an E2-conjugating enzyme (UFC1), and an E3-ligation enzyme (UFL1). Several substrates for UFMylation have been identified including DDRGK1, RPL26, p53, MRE11, histone H4, ASC1, CDK5RAP3, and CYB5R3 (5-12). Modification of these targets by UFM1 has direct effects on endoplasmic reticulum (ER) homeostasis, protein translation, and response to DNA damage. Notably, independent screens identified UFMylation as a key regulator of ER-phagy and autophagy (5,13,14). DDRGK1 and RPL26 have been described as key UFM1 substrates regulating ER homeostasis (5,15). Aberrant UFMylation is associated with many pathological conditions, including cancer, diabetes, and inflammatory diseases (2-4).
    1. Tatsumi, K. et al. (2010) J Biol Chem 285, 5417-27.
    2. Gerakis, Y. et al. (2019) Trends Cell Biol 29, 974-986.
    3. Jiang, Q. et al. (2023) Front Endocrinol (Lausanne) 14, 1123124.
    4. Jing, Y. et al. (2022) Cancers (Basel) 14, 3501. doi: 10.3390/cancers14143501.
    5. Liu, J. et al. (2017) Nat Commun 8, 14186.
    6. Walczak, C.P. et al. (2019) Proc Natl Acad Sci USA 116, 1299-1308.
    7. Liu, J. et al. (2020) Nat Cell Biol 22, 1056-1063.
    8. Lee, L. et al. (2021) Sci Adv 7, eabc7371.
    9. Qin, B. et al. (2019) Nat Commun 10, 1242.
    10. Yoo, H.M. et al. (2014) Mol Cell 56, 261-274.
    11. Yang, R. et al. (2019) Development 146, dev169235. doi: 10.1242/dev.169235.
    12. Ishimura, R. et al. (2022) Nat Commun 13, 7857.
    13. Liang, J.R. et al. (2020) Cell 180, 1160-1177.e20.
    14. DeJesus, R. et al. (2016) Elife 5, e17290. doi: 10.7554/eLife.17290.
    15. Scavone, F. et al. (2023) Proc Natl Acad Sci USA 120, e2220340120.

    Database Links

    UniProt ID: P61254 , O94874 , P61960 , Q96HY6


    Entrez-Gene Id: 6154 , 23376 , 51569 , 65992


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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