Render Target: STATIC
Render Timestamp: 2024-11-21T13:54:19.443Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:43.567
Product last modified at: 2024-10-14T18:30:08.060Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

uPA (E2M6I) Rabbit mAb #15800

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 32, 50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    uPA (E2M6I) Rabbit mAb recognizes endogenous levels of total uPA protein. The antibody detects the 50 kDa zymogen and the 32 kDa chain B, and does not detect chain A.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser266 of human uPA protein.

    Background

    uPA (PLAU, urokinase-type plasminogen activator) is a secreted serine protease that plays an important role in plasminogen activation (1). uPA exists as a secreted 50 kDa inactive zymogen. Upon binding to its high affinity serine protease urokinase-type plasminogen activator receptor (uPAR), it is processed to two interlinked chains, 18 kDa chain A and 32 kDa chain B, and becomes activated to convert plasminogen to its active form, plasmin, in a spatially restricted manner on the cell surface (2). Plasmin then carries out its protease activity toward a wide range of substrates. uPA activity is inhibited by serpins (serine protease inhibitors), such as plasminogen activator inhibitor (PAI) (3). uPA, uPAR, and PAI form a Urokinase Plasminogen Activation System (uPAS) (4). This system plays a key role in regulating a broad range of biological processes under normal physiological conditions, such as tissue wound healing (5), cell migration (6), fibrinolysis (7), and angiogenesis (8). The system also contributes to disease progression, such as cancer development (9), neurodegeneration disorders (10), inflammatory lung disease, and rheumatoid arthritis (11,12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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