Render Target: STATIC
Render Timestamp: 2024-12-26T11:27:00.172Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:29:10.747
Product last modified at: 2024-12-11T20:45:10.040Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

UPF1 Antibody #9435

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 140
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    UPF1 Antibody detects endogenous levels of total UPF1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UPF1. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    UPF1 was identified as an active component in nonsense-mediated mRNA decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). UPF1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). UPF1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, UPF2 and UPF3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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