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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. USP Antibody Sampler Kit

USP Antibody Sampler Kit #12894

Inquiry Info. # 12894

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    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The USP Antibody Sampler Kit provides an economical means of detecting members of the ubiquitin-specific protease (USP) family. The kit includes enough primary antibody to perform two western blot experiments per primary antibody.

    Background

    Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). The ubiquitin-specific protease (USP) subfamily is one of five distinct groups of DUB enzymes. Ubiquitin-specific-processing protease 1 (USP1) is regulated in a cell cycle dependent manner by both transcriptional and ubiquitin-proteasomal mechanisms (3). Nuclear USP1 localizes to chromatin where it deubiquitinates monoubiquitinated FANCD2 and plays an important role in DNA damage repair and Chk1 protein stability (3,4). Ubiquitin-specific-processing protease 2 (USP2) contains C19 peptidase activity and is involved in ubiquitin recycling and disassembly of polymeric ubiquitin and ubiquitin-like protein complexes (5). USP2 is a putative oncoprotein that is highly over expressed in prostate cancer and drives tumor growth by binding and stabilizing fatty acid synthase through deubiquitination (6,7).

    Herpesvirus-associated ubiquitin-specific protease (HAUSP, USP7) binds and deubiquitinates transcription factor p53 and regulator protein Mdm2, stabilizing both proteins (8,9). HAUSP modifies other ubiquitinated proteins, including FoxO family forkhead transcription factors and the mitotic stress checkpoint protein CHFR (10,11). Ubiquitin-specific protease 8 (USP8, UBPy) is a cysteine protease and growth-regulated enzyme that is essential for cell proliferation and survival (12,13). The catalytic domain of USP9X possesses cysteine peptidase activity that cleaves ubiquitin and polyubiquitin conjugates. USP9X may help stabilize adherens and tight junction molecules during epithelial cell polarization (14,15). USP10 is regulated at the posttranslational level through protein-protein interactions and phosphorylation. Interaction of USP10 with the Ras-GAP SH3 domain binding protein (G3BP) inhibits the ability of USP10 to catalyze ubiquitin chain disassembly (16). ATM-mediated phosphorylation of USP10 at Thr42 and Ser337 promotes USP10 stabilization and relocation from the cytoplasm to the nucleus, where it functions in p53 deubiquitination, stabilization, and activation in response to genotoxic stress (17).

    USP14 is recruited to the proteasome through association with the PSMD2 (S2/hRPN1) subunit of the 19S regulatory particle, where it may antagonize substrate degradation (18,19). USP14 trims ubiquitin residues from distal polyubiquitin chain ends, decreasing chain affinity for proteasome ubiquitin receptors and allowing for enhanced substrate stability (20,21). USP18 (UBP43) catalyzes the removal of the interferon-regulated, ubiquitin-like protein ISG15 from conjugated proteins (22). Removal of ISG15 from target proteins maintains a critical balance of cellular ISG15-conjugated proteins, which is important for normal development and brain function (23,24). USP28 can bind, deubiquitinate and stabilize several DNA-damage pathway proteins, including p53BP1 and Chk2 (25). USP28 plays an important role in Myc-related signaling as it catalyzes Myc deubiquitination and promotes Myc stabilization, which contributes to tumor-cell growth (26).
    1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
    2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
    3. Nijman, S.M. et al. (2005) Mol Cell 17, 331-9.
    4. Guervilly, J.H. et al. (2011) Hum Mol Genet 20, 2171-81.
    5. Wilkinson, K.D. (1997) FASEB J 11, 1245-56.
    6. Graner, E. et al. (2004) Cancer Cell 5, 253-61.
    7. Priolo, C. et al. (2006) Cancer Res 66, 8625-32.
    8. Li, M. et al. (2002) Nature 416, 648-53.
    9. Brooks, C.L. et al. (2007) Oncogene 26, 7262-6.
    10. van der Horst, A. et al. (2006) Nat Cell Biol 8, 1064-73.
    11. Oh, Y.M. et al. (2007) Biochem Biophys Res Commun 357, 615-9.
    12. Naviglio, S. et al. (1998) EMBO J 17, 3241-50.
    13. Niendorf, S. et al. (2007) Mol Cell Biol 27, 5029-39.
    14. Murray, R.Z. et al. (2004) Mol Biol Cell 15, 1591-9.
    15. Théard, D. et al. (2010) EMBO J 29, 1499-509.
    16. Soncini, C. et al. (2001) Oncogene 20, 3869-79.
    17. Yuan, J. et al. (2010) Cell 140, 384-96.
    18. Lee, B.H. et al. (2010) Nature 467, 179-84.
    19. Koulich, E. et al. (2008) Mol Biol Cell 19, 1072-82.
    20. Hanna, J. et al. (2006) Cell 127, 99-111.
    21. Thrower, J.S. et al. (2000) EMBO J 19, 94-102.
    22. Malakhov, M.P. et al. (2002) J Biol Chem 277, 9976-81.
    23. Rempel, L.A. et al. (2007) Reprod Biol Endocrinol 5, 13.
    24. Ritchie, K.J. et al. (2002) Genes Dev 16, 2207-12.
    25. Zhang, D. et al. (2006) Cell 126, 529-42.
    26. Popov, N. et al. (2007) Nat Cell Biol 9, 765-74.

    Database Links

    UniProt ID: Q93008 , O75604 , P40818 , O94782 , P54578 , Q9UMW8 , Q96RU2 , Q93009 , Q14694


    Entrez-Gene Id: 8239 , 9099 , 9101 , 7398 , 9097 , 11274 , 57646 , 7874 , 9100


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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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