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UTX (D3Q1I) Rabbit mAb #33510

Filter:
  • WB
  • IP
  • IHC
Western blot analysis of extracts from various cell lines using UTX (D3Q1I) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, THP-1 cells are negative for UTX.

To Purchase # 33510

Cat. # Size Qty. Price
33510T 20 µl
$167
33510S 100 µl
$339

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 180
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunohistochemistry (Paraffin) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

UTX (D3Q1I) Rabbit mAb recognizes endogenous levels of total UTX protein. This antibody does not cross-react with UTY protein. This antibody also cross-reacts with unidentified proteins of 60 kDa and 70 kDa.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Ala490 of human UTX protein.

Background

The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). The three members of the UTX/UTY family include the ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX), the ubiquitously transcribed Y chromosome tetratricopeptide repeat protein (UTY), and JmjC domain-containing protein 3 (JMJD3) (3). This family of proteins has been shown to demethylate both di- and tri-methyl histone H3 Lys 27 (4-8). The UTX gene escapes X inactivation in females and is ubiquitously expressed (9). UTX functions to regulate HOX gene expression during development (4-6). JMJD3 functions to regulate gene expression in macrophages responding to various inflammatory stimuli and has been shown to be upregulated in prostate cancer (7,8). Both UTX and JMJD3 interact with mixed-lineage leukemia (MLL) complexes 2 and 3, both of which have been shown to methylate histone H3 at Lys4 (6,7). The UTY gene is expressed in most tissues in the male mouse (10).

Alternate Names

de-methylase; demethylase; KDM6A

Pathways

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