Render Target: STATIC
Render Timestamp: 2024-12-20T10:44:08.186Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-20 22:01:09.774
Product last modified at: 2024-12-17T22:45:08.466Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

VASP (9A2) Rabbit mAb #3132

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R Hm Mk B
    SENSITIVITY Endogenous
    MW (kDa) 46, 50
    Source/Isotype Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Mk-Monkey 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:500
    Immunofluorescence (Frozen) 1:200
    Immunofluorescence (Immunocytochemistry) 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VASP (9A2) Rabbit mAb detects endogenous levels of total VASP protein.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Monkey, Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human and mouse VASP.

    Background

    Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation, and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site, while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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