Render Target: STATIC
Render Timestamp: 2024-12-18T10:48:47.247Z
Commit: ff25cf0788e69a87df3da505ebb7b292b97eec1a
XML generation date: 2024-04-05 20:22:58.382
Product last modified at: 2024-05-30T07:08:40.253Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

VCAM-1 Antibody #12367

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Inquiry Info. # 12367

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    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 95-120
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VCAM-1 Antibody recognizes endogenous levels of total VCAM-1 protein. This antibody cross-reacts with proteins of unknown origin 48 and 49 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro354 of human VCAM-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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