Render Target: STATIC
Render Timestamp: 2024-12-20T10:54:59.768Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-20 06:18:59.825
Product last modified at: 2024-11-27T14:15:14.707Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

VCIP135 Antibody #88153

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 140
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VCIP135 Antibody recognizes endogenous levels of total VCIP135 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1035 of human VCIP135 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes, respectively (1,2). Deubiquitinating enzymes (DUBs) are categorized into five subfamilies based on catalytic domain structure: USP, OTU, MJD, and JAMM. The valosin-containing protein p97/p47 complex-interacting protein 1 (VCIP135, VCPIP1) is a deubiquitinating enzyme that belongs to the A20-like subfamily of ovarian tumor (OTU) DUBs (3). VCIP135 serves as a cofactor for the p97/p47 complex in regulating Golgi membrane fusion and reassembly at the end of mitosis (4-6). Research studies suggest that the phosphorylation status of VCIP135 provides a mechanism to fine-tune the kinetics of Golgi disassembly and reassembly during the cell cycle. For example, these studies demonstrate that VCIP135 undergoes phosphorylation early in mitosis, which blocks its association with the Golgi membrane and p97/VCP, thus inhibiting p97/VCP-mediated Golgi membrane fusion (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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