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Primary Antibodies /
VEGF-A Antibody

VEGF-A Antibody #65373

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Product Name

50661 VEGF-A (E9X8Q) Rabbit mAb

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Inquiry Info. # 65373

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	16, 20, 23, 26
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

VEGF-A Antibody recognizes endogenous levels of all isoforms of total VEGF-A protein. This antibody also detects a 47 kDa band of unknown origin.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His38 of human VEGF-A protein. Antibodies are purified by peptide affinity chromatography.

Background

Vascular endothelial growth factor-A (VEGF-A; formerly VEGF) belongs to the VEGF/PDGF family of growth factors that play a critical role in promoting vascular development. VEGF-A is primarily known for its function in promoting angiogenesis, the process by which new blood vessels are sprouted from pre-existing blood vessels. VEGF-A has eight isoforms, derived from differentially spliced mRNA variants encoded by the VEGF-A gene (1). All isoforms contain a conserved receptor binding domain, but differ in their C-terminal domains that regulate binding to components in the extracellular matrix. VEGF-A is secreted by multiple cell types, including fibroblasts, macrophages, and some tumor cells (1). Binding of VEGF-A to its cognate receptors (VEGFR2 and to a lesser extent VEGFR1) on the endothelial cell surface activates the receptor and initiates downstream signaling, resulting in endothelial cell proliferation, survival, migration, and changes to vascular permeability, all crucial processes for angiogenesis (3). Tumor cells have been shown to respond to hypoxia by increasing VEGF-A mRNA expression. Increased secretion of VEGF-A in the tumor microenvironment stimulates angiogenesis to further promote tumor progression (4). In addition to promoting tumor angiogenesis, VEGF-A has also been shown to play an important role in normal tissue development, tissue regeneration, tumor cell proliferation, neovascular eye diseases, and cancer immunity (2,5). For this reason, targeting angiogenesis via the VEGF-A pathway has been a major focus in cancer therapeutics research.

Database Links

UniProt ID: P15692-1

Entrez-Gene Id: 7422

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For Research Use Only. Not For Use In Diagnostic Procedures.

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