Render Target: STATIC
Render Timestamp: 2025-03-14T10:44:03.010Z
Commit: a619ae74f66dae0f27639e88da12bcf600e46428
XML generation date: 2025-03-07 13:19:55.637
Product last modified at: 2025-02-20T14:15:11.422Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

VGAT (F8M4M) Rabbit mAb #77460

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 57
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Frozen) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VGAT (F8M4M) Rabbit mAb recognizes endogenous levels of total VGAT protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human VGAT protein.

    Background

    The vesicular GABA transporter (VGAT), also known as the vesicular inhibitory amino acid transporter (VIAAT), loads GABA and glycine from the neuronal cytoplasm into synaptic vesicles for vesicular storage and subsequent exocytosis of these inhibitory amino acids. VGAT is present in both GABAergic and glycinergic neurons, with its expression limited to the brain (1,2). It can also recognize β-alanine as a substrate (2).

    Recently, tau accumulation, as found in Alzheimer’s disease (AD) patients, has been shown to selectively suppress the expression of the intracellular VGAT (3). At the same time, VGAT activation can help with anxiety exacerbated by AD pathology (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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