Render Target: STATIC
Render Timestamp: 2025-03-07T12:38:46.530Z
Commit: a619ae74f66dae0f27639e88da12bcf600e46428
XML generation date: 2024-08-01 15:31:04.288
Product last modified at: 2024-05-30T07:09:51.360Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

VGLUT1 Antibody #12331

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Inquiry Info. # 12331

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    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 62
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VGLUT1 Antibody recognizes endogenous levels of total VGLUT1 protein.

    Species Reactivity:

    Mouse, Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human VGLUT1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Glutamatergic neurons release glutamate, the most common excitatory neurotransmitter. Their synaptic vesicles are filled with glutamate by vesicular glutamate transporters, VGLUTs (1). VGLUT1, also called solute carrier family 17 member 7 (SLC17A7), was first identified as an inorganic phosphate transporter (2). Despite the absence of homology with neurotransmitter transporters, VGLUT1 was later demonstrated to be a glutamate transporter (1) specific to glutamatergic neurons (3). Closely related to VGLUT1, VGLUT2 and VGLUT3 are also involved in glutamate uptake into synaptic vesicles, but define different neuronal subpopulations (4,5). VGLUT1 and VGLUT2 are the most abundant isoforms. VGLUT1 is expressed in the cortex, hippocampus, and cerebellar cortex, while VGLUT2 is mostly found in the thalamus (6,7). VGLUT3 is expressed in hair cells of the auditory system (8).
    Research studies have linked VGLUT1 to schizophrenia (9) and depressive disorders (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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