VGLUT1 (E9D2B) Mouse mAb (BSA and Azide Free) #34007
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| Source/Isotype | Mouse IgG2b kappa |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
VGLUT1 (E9D2B) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total VGLUT1 protein. This antibody does not cross-react with other VGLUT proteins. This antibody detects a 45 kDa band of unknown origin in human cerebellum lysate.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg40 of human VGLUT1 protein.
Background
Glutamatergic neurons release glutamate, the most common excitatory neurotransmitter. Their synaptic vesicles are filled with glutamate by vesicular glutamate transporters, VGLUTs (1). VGLUT1, also called solute carrier family 17 member 7 (SLC17A7), was first identified as an inorganic phosphate transporter (2). Despite the absence of homology with neurotransmitter transporters, VGLUT1 was later demonstrated to be a glutamate transporter (1) specific to glutamatergic neurons (3). Closely related to VGLUT1, VGLUT2 and VGLUT3 are also involved in glutamate uptake into synaptic vesicles, but define different neuronal subpopulations (4,5). VGLUT1 and VGLUT2 are the most abundant isoforms. VGLUT1 is expressed in the cortex, hippocampus, and cerebellar cortex, while VGLUT2 is mostly found in the thalamus (6,7). VGLUT3 is expressed in hair cells of the auditory system (8).
- Bellocchio, E.E. et al. (2000) Science 289, 957-60.
- Ni, B. et al. (1996) J Neurochem 66, 2227-38.
- Takamori, S. et al. (2000) Nature 407, 189-94.
- Fremeau, R.T. et al. (2001) Neuron 31, 247-60.
- Fremeau, R.T. et al. (2002) Proc Natl Acad Sci U S A 99, 14488-93.
- Herzog, E. et al. (2001) J Neurosci 21, RC181.
- Kaneko, T. and Fujiyama, F. (2002) Neurosci Res 42, 243-50.
- Seal, R.P. et al. (2008) Neuron 57, 263-75.
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