Render Target: STATIC
Render Timestamp: 2024-12-20T11:19:43.590Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:54:59.115
Product last modified at: 2024-12-17T18:52:27.644Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

VRK1 (1F6) Mouse mAb #3307

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 48
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VRK1 (1F6) Mouse mAb detects endogenous levels of total VRK1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant fusion protein GST-full length human VRK1.

    Background

    The vaccinia-related kinase (VRK) proteins are a new group of Ser/Thr kinases in the human kinome. This mammalian kinase family comprises three members, VRK1, VRK2, and VRK3 (1-3). The VRK1 has autophosphorylation activity and phosphorylates several transcription factors, including p53 (4), ATF2 (5), and c-Jun (6). VRK2 is associated with the endoplasmic reticulum (7). VRK3 suppresses Erk activity through direct interaction and activation of the MAP kinase phosphatase VHR (8). Further functional and structural analysis of VRK proteins will elucidate important new aspects of cell regulation.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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