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VSV-G Tag Antibody

VSV-G Tag Antibody #81454

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IF-Immunofluorescence

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunofluorescence (Immunocytochemistry)	1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

VSV-G Tag Antibody detects recombinant proteins containing the VSV-G epitope tag. The antibody recognizes the VSV-G tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects a non-specific band of unknown origin at 65 kDa.

Species Reactivity:

All Species Expected

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide containing the vesicular stomatitis virus glycoprotein epitope (YTDIEMNRLGK). Antibodies are purified by peptide affinity chromatography.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The VSV-G tag is derived from an epitope from the C-terminal region of vesicular stomatitis virus glycoprotein (1). VSV-G is widely used both as an epitope tag in expression vectors and for pseudotyping lentiviral vectors due to the stability and broad host range of the resulting pseudotypes (2,3).

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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