Render Target: STATIC
Render Timestamp: 2024-12-20T11:02:12.328Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-25 16:36:52.190
Product last modified at: 2024-12-03T11:15:25.844Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

WDR4 (F1T3E) Rabbit mAb #67470

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    WDR4 (F1T3E) Rabbit mAb recognizes endogenous levels of total WDR4 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro405 of human WDR4 protein.

    Background

    WD repeat domain 4 (WDR4) protein forms a complex with METTL1 to catalyze 7-methylguanosine (m7G) on mRNA, tRNA, and miRNAs (1,2). METTL1 and WDR4 are required for m7G modification, with METTL1 being the catalytic component and WDR4 stabilizing the complex (2). m7G on mRNA is required for translation and stem cell differentiation, while m7G on miRNA can regulate structure and biogenesis (3,4). WDR4 mutations impair the methylation of m7G46 of tRNA species and can cause primordial dwarfism and neurological symptoms (5-7). Overexpression of WDR4 has been shown in hepatocellular carcinoma, resulting in increased m7G levels and inhibition of apoptosis (8). Furthermore, WDR4 is associated with immune infiltration in various tumors, making it a potential target for immunotherapy (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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