WDR5 (D3X5B) Rabbit mAb #13073
- WB
To Purchase # 13073
| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 13073T | 20 µl | $153 | |
| 13073S | 100 µl | $339 |
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 37 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
WDR5 is a core subunit of all SET1/MLL histone methyltransferase complexes and is required for proper complex assembly and histone methyltransferase activity (7). It functions as an effector of histone H3 Lys4 methylation by recruiting SET1/MLL complexes to target loci and presenting the histone H3 amino-terminal tail for methylation (8). WDR5 contains a classical, seven-bladed WD40 propeller domain with a central cavity that binds to histone H3 Arg2 when symmetrically di-methylated (H3Arg2Me2-S) by arginine methyltransferases PRMT5 and PRMT7 (8). WDR5 binding to H3Arg2Me2-S results in increased recruitment of SET1/MLL complexes and methylation of histone H3 Lys4 at gene promoters and distal regulatory sites. In contrast, asymmetric di-methylation of histone H3 Arg2 (H3Arg2Me2-A) by PRMT6 reduces WDR5 binding and results in decreased recruitment of SET1/MLL complexes and reduced histone H3 Lys4 methylation (8). Interestingly, the H3Arg2Me2-S binding pocket of WDR5 also interacts with the SET domains of SET1/MLL proteins with comparable affinity, setting up a potential competition for WDR5 binding that may act to regulate SET1/MLL recruitment and subsequent H3 Lys4 methylation (9-11). WDR5 is also a core subunit of the ATAC and MOF-NSL histone acetyltransferase complexes and the CHD8 chromatin-remodeling complex (12-14).
- Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
- Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.
- Migliori, V. et al. (2012) Epigenetics 7, 815-22.
- Migliori, V. et al. (2012) Nat Struct Mol Biol 19, 136-44.
- Song, J.J. and Kingston, R.E. (2008) J Biol Chem 283, 35258-64.
- Patel, A. et al. (2008) J Biol Chem 283, 32162-75.
- Zhang, P. et al. (2012) Nucleic Acids Res 40, 4237-46.
- Wang, Y.L. et al. (2008) J Biol Chem 283, 33808-15.
- Cai, Y. et al. (2010) J Biol Chem 285, 4268-72.
- Thompson, B.A. et al. (2008) Mol Cell Biol 28, 3894-904.
Limited Uses
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