WDR82 (D2I3B) Rabbit mAb #99715
- WB
- IP
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 30 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
WDR82 is a core subunit specific to the SET1A and SET1B COMPASS-like complexes (4,5). WDR82 facilitates methylation of histone H3 lysine 4 during transcriptional activation by binding to Ser5-phosphorylated Rpb1 CTD and ubiquityl-H2B, and recruiting SET1A/SET1B complexes to the transcription start site of genes (7,8). WDR82 is also a component of the PTW/PP1 phosphatase complex that functions to regulate chromatin structure and cell cycle progression during the transition from mitosis to interphase (9).
- Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
- Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.
- Lee, J.H. and Skalnik, D.G. (2008) Mol Cell Biol 28, 609-18.
- Wu, M. et al. (2008) Mol Cell Biol 28, 7337-44.
- Lee, J.H. et al. (2010) J Biol Chem 285, 24466-76.
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