Whitlow/218 Linker (E3U7Q) Rabbit mAb #57710

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
F-Flow Cytometry

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Description

This Cell Signaling Technology antibody is tested in-house for indirect flow cytometric analysis in human cells and is expected to react with cell surface expressed CARs of varying specificity, which contain a Whitlow/218 linker within the scFv of the extracellular domain.

Product Usage Information

Application	Dilution
Simple Western™	1:10
Flow Cytometry (Live)	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #66159.

Protocol

Specificity / Sensitivity

Whitlow/218 Linker (E3U7Q) Rabbit mAb recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker.
This antibody is not approved for traditional Western blot analysis.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide.

Background

The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1).

Whitlow, M. et al. (1993) Protein Eng 6, 989-95.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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