Render Target: STATIC
Render Timestamp: 2025-01-02T12:21:57.488Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-09-30 01:53:55.769
Product last modified at: 2025-01-01T09:06:43.278Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

XAF1 (E1E4O) Rabbit mAb #13805

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 32, 38
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    XAF1 (E1E4O) Rabbit mAb recognizes endogenous levels of total XAF1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val210 of human XAF1 protein.

    Background

    X-linked inhibitor of apoptosis (XIAP)-associated factor 1 (XAF1) is a zinc finger protein that antagonizes the anti-apoptotic activity of XIAP (1,2). XIAP, a member of the inhibitor of apoptosis (IAP) family, inhibits apoptosis by direct inhibition of caspases (3; reviewed in 4). XAF1 is widely expressed in normal tissues, with highest levels in the heart and ovary, but is mostly reduced in cancer lines (1,2). Expression of XAF1 can be induced by interferons via Stat transcriptional activity (5-7). The levels of XAF1 have been shown to be inversely correlated with p53, and p53 is directly responsible for inhibiting XAF1 transcription (8,9). A number of studies have shown that XAF1 can function as a tumor suppressor protein, and decreased levels of XAF1 are found in a variety of different cancers (10-13). Research studies suggest that expression of XAF1 may be a prognostic biomarker for some cancers (14-16).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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