Render Target: STATIC
Render Timestamp: 2024-12-26T11:25:24.568Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:54.824
Product last modified at: 2024-12-17T18:52:59.539Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Xanthine Dehydrogenase/Oxidase (E2U8Y) Rabbit mAb #35792

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 146
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Xanthine Dehydrogenase/Oxidase (E2U8Y) Rabbit mAb recognizes endogenous levels of total xanthine dehydrogenase/oxidase protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu560 of human xanthine dehydrogenase/oxidase protein.

    Background

    Xanthine dehydrogenase/oxidase is a rate-limiting enzyme in purine degradation. It catalyzes the oxidation of hypoxanthine to xanthine and the oxidation of xanthine to uric acid. The circadian rhythm of the expression of this enzyme and its enzymatic activity lead to circadian oscillation of uric acid concentrations in the liver and blood (1). In macrophages, the histone methyltransferase Setdb2 mediates the elevated expression of xanthine dehydrogenase/oxidase, and consequently, the increased production of uric acid. Pharmacological targeting of the uric acid pathway of purine degradation improves wound healing (2). In addition, research studies show that the xanthine oxidase activity of this enzyme contributes to the reactive oxygen species generation in macrophages and is essential in innate inflammatory signaling (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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