Render Target: STATIC
Render Timestamp: 2024-10-30T09:56:50.459Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-04-05 20:38:08.366
Product last modified at: 2024-10-25T12:00:13.334Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

XRN1 Antibody #70205

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 200
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    XRN1 Antibody recognizes endogenous levels of total XRN1 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1696 of human XRN1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    5’-3’ exoribonuclease 1 (XRN1) is a cytoplasmic exonuclease that degrades RNA containing a 5’-monophosphate to component mononucleotides. XRN1 is the primary exonuclease associated with ribosomes in the cytoplasm and is responsible for mRNA turnover (1,2). This turnover is facilitated in discrete structures in the cytoplasm called P-bodies that contain decapping and deadenylation proteins (3). XRN1 also plays a role in RISC-mediated mRNA degradation, as it associates with 3’ mRNA fragments generated by RISC cleavage. This process does not require uncapping or deadenylation (4). XRN1 plays a significant role in viral RNA degradation (5). As such, many viral genomes, including hepatitis C, Dengue, and West Nile, encode for XRN1-resistant long non-coding RNA that affect innate immunity and viral replication (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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