Render Target: STATIC
Render Timestamp: 2024-12-26T11:41:59.862Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-29 22:01:06.661
Product last modified at: 2024-12-04T14:00:26.433Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Zap-70 (L1E5) Mouse mAb #2709

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 70
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #59806.

    Protocol

    Specificity / Sensitivity

    Zap-70 (L1E5) Mouse mAb detects endogenous levels of total Zap-70 protein. It does not cross-react with other related proteins.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the Ser289 of human Zap-70.

    Background

    The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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