Render Target: STATIC
Render Timestamp: 2024-11-21T13:28:27.987Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:17:46.133
Product last modified at: 2024-11-18T21:15:09.256Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

ZBP1 Antibody #60968

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 44, 58
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZBP1 Antibody recognizes endogenous levels of total ZBP1 protein. A band of unknown identity is detected at around 19 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln372 of human ZBP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide binding protein that plays a role in tumorigenesis and innate immune responses to viral infection (1). It is expressed at high levels in lymphatic tissues and intestine and is induced in macrophages by interferon gamma or by LPS (2,3). It contains two amino-terminal Z-alpha domains that bind to left-handed Z-DNA and Z-RNA (4,5). In addition, an adjacent domain binds right-handed B-DNA that allows for it to function as a cytosolic DNA sensor in innate immune responses, triggering activation of TBK1 and IRF-3, and subsequent production of type I interferons (6,7). Furthermore, ZBP1 can trigger the activation of NF-κB through interaction with the RIP homotypic interaction motif (RHIM) of RIPK1 and RIPK3 (8). ZBP1 binding to RIPK3 can also induce a process of programmed necrosis termed necroptosis (9). In contrast, its interaction with RIPK1 can repress necroptosis (10,11). The mRNA binding activity of ZBP1 is also thought to play a role in tumorigenesis. ZBP1 is repressed in metastatic breast cancer, which leads to dysregulation of mRNA targets involved in proliferation and metastasis (12,13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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