Render Target: STATIC
Render Timestamp: 2024-12-20T10:39:49.283Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:35.871
Product last modified at: 2024-12-17T18:49:37.185Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ZBTB24 (E5D6G) Rabbit mAb #15969

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 95
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Chromatin IP 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZBTB24 (E5D6G) Rabbit mAb recognizes endogenous levels of total ZBTB24 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro569 of human ZBTB24 protein.

    Background

    Zinc finger and BTB domain-containing protein 24 (ZBTB24), also called ZNF450, is a DNA-binding protein initially found to be expressed in response to BMP-2 signaling (1). ZBTB24 along with CDCA7 play a role in DNA methylation by recruiting the HELLS chromatin remodeling complex to centromeric satellite repeats (2). Mutations in ZBTB24 are linked to immunodeficiency and centromeric instability, and facial anomalies syndrome type 2 (ICF2) (3,4). ZBTB24 is recruited to DNA breaks by PARP1 and promotes assembly of the LIG4/XRCC4 complex, resulting in error free non-homologous end joining (NHEJ) (5). ZBTB24 has also been shown to play a role in trophoblast proliferation through the methylation of E-cadherin (6)
    For Research Use Only. Not For Use In Diagnostic Procedures.
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