Render Target: STATIC
Render Timestamp: 2024-12-30T11:42:21.926Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:55.526
Product last modified at: 2024-12-23T12:15:34.254Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ZFP36L1 (E6L6S) Rabbit mAb #30894

Filter:
  • WB

    Supporting Data

    REACTIVITY H M Mk
    SENSITIVITY Endogenous
    MW (kDa) 45-50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZFP36L1 (E6L6S) Rabbit mAb recognizes endogenous levels of total ZFP36L1 protein. This antibody does not cross-react with ZFP36L2 protein.

    Species Reactivity:

    Human, Mouse, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro281 of human ZFP36L1 protein.

    Background

    ZFP36L1, also known as butyrate response factor-1 (BRF1), and ZFP36L2, also known as butyrate response factor-2 (BRF2), both belong to the TIS11 family of CCCH zinc-finger proteins (1). This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (AREs) found in the 3'-untranslated regions of mRNAs and promote deadenylation and rapid degradation by the exosome (2,3). These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors, and cell cycle regulators, including GM-CSF, TNFα, IL-2, IL-3, and IL-6 (4,5). Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation (6-8). Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of ZFP36L1 at Ser92, which results in binding by 14-3-3 protein and inactivation of ZFP36L1 (9). ZFP36L1 and L2 have also been shown to promote cell quiescence in developing B lymphocytes, promoting VDJ recombination (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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