ZFP36L1 (E6L6S) Rabbit mAb #30894
- WB
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro281 of human ZFP36L1 protein.
Background
ZFP36L1, also known as butyrate response factor-1 (BRF1), and ZFP36L2, also known as butyrate response factor-2 (BRF2), both belong to the TIS11 family of CCCH zinc-finger proteins (1). This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (AREs) found in the 3'-untranslated regions of mRNAs and promote deadenylation and rapid degradation by the exosome (2,3). These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors, and cell cycle regulators, including GM-CSF, TNFα, IL-2, IL-3, and IL-6 (4,5). Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation (6-8). Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of ZFP36L1 at Ser92, which results in binding by 14-3-3 protein and inactivation of ZFP36L1 (9). ZFP36L1 and L2 have also been shown to promote cell quiescence in developing B lymphocytes, promoting VDJ recombination (10).
- Varnum, B.C. et al. (1991) Mol. Cell. Biol. 11, 1754-1758.
- Stoecklin, G. et al. (2002) EMBO J. 21, 4709-4718.
- Lykke-Andersen, J. and Wagner, E. (2005) Genes Dev. 19, 351-361.
- Stoecklin, G. et al. (2000) Mol. Cell. Biol. 20, 3753-3763.
- Stoecklin, G. et al. (2001) RNA 7, 1578-1588.
- Schuler, G.D. and Cole, M.D. (1988) Cell 55, 1115-1122.
- Nair, A.P. et al. (1994) Nature 369, 239-242.
- Carballo, E. et al. (1998) Science 281, 1001-1005.
- Schmidlin, M. et al. (2004) EMBO J. 23, 4760-4769.
- Galloway, A. et al. (2016) Science 352, 453-9.
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