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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ZFP36L1 (E6L6S) Rabbit mAb #30894

Filter:
  • WB
Western Blotting Image 1: ZFP36L1 (E6L6S) Rabbit mAb
Western blot analysis of extracts from various cell lines using ZFP36L1 (E6L6S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Low expression of ZFP36L1 protein in OVCAR-4 cells is consistent with the predicted expression pattern.

To Purchase # 30894

Cat. # Size Qty. Price
30894T 20 µl
$153
30894S 100 µl
$339

Supporting Data

REACTIVITY H M Mk
SENSITIVITY Endogenous
MW (kDa) 45-50
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ZFP36L1 (E6L6S) Rabbit mAb recognizes endogenous levels of total ZFP36L1 protein. This antibody does not cross-react with ZFP36L2 protein.

Species Reactivity:

Human, Mouse, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro281 of human ZFP36L1 protein.

Background

ZFP36L1, also known as butyrate response factor-1 (BRF1), and ZFP36L2, also known as butyrate response factor-2 (BRF2), both belong to the TIS11 family of CCCH zinc-finger proteins (1). This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (AREs) found in the 3'-untranslated regions of mRNAs and promote deadenylation and rapid degradation by the exosome (2,3). These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors, and cell cycle regulators, including GM-CSF, TNFα, IL-2, IL-3, and IL-6 (4,5). Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation (6-8). Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of ZFP36L1 at Ser92, which results in binding by 14-3-3 protein and inactivation of ZFP36L1 (9). ZFP36L1 and L2 have also been shown to promote cell quiescence in developing B lymphocytes, promoting VDJ recombination (10).
For Research Use Only. Not For Use In Diagnostic Procedures.
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