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Render Timestamp: 2024-12-26T12:12:08.854Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:22.102
Product last modified at: 2024-12-17T18:54:45.541Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. ZHX2 (E5H5G) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ZHX2 (E5H5G) Rabbit mAb #43035

Filter:
  • WB
  • IP
  • IF
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZHX2 (E5H5G) Rabbit mAb recognizes endogenous levels of total ZHX2 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ZHX2 protein.

    Background

    Zinc fingers and homeoboxes protein 2 (ZHX2) is a transcriptional repressor and tumor suppressor that belongs to the zinc fingers and homeoboxes (ZHX) protein family, which also contains ZHX1 and ZHX3 (1). ZHX proteins are expressed only in vertebrates and contain two C2H2 zinc finger motifs as well as five homeodomains, which are involved in DNA-protein and protein-protein interactions (2). ZHX2 has been shown to form homodimers as well as heterodimers with ZHX1, and also interacts with nuclear factor Y subunit alpha (NF-YA) (2). Through these interactions, ZHX2 functions as a transcriptional repressor by reducing the expression levels of a number of genes including cyclin A, cyclin E, and alpha-fetoprotein, among others (2-4). ZHX2 functions as a tumor suppressor in some tissues, as loss of ZHX2 leads to hepatocellular carcinoma, lymphoma, and myeloma (4). In clear cell renal carcinoma (ccRCC), ZHX2 appears to function as an oncogene, as loss of von Hippel-Lindau protein (VHL) leads to increased expression of ZHX2 and increased cellular proliferation (5). ZHX2 also contributes to atherosclerosis by promoting macrophage survival and pro-inflammatory function, leading to growth of atherosclerotic lesions (6). Additionally, ZHX2 functions to promote the maintenance of neural progenitor cells in the brain through repression of ephrin-B (7).

    Database Links

    UniProt ID: Q9Y6X8


    Entrez-Gene Id: 22882


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
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