Render Target: STATIC
Render Timestamp: 2024-12-26T12:00:18.379Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 02:00:06.662
Product last modified at: 2024-09-30T08:02:30.494Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. ZNF366 (E5O9G) Rabbit mAb (ChIP Formulated)
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ZNF366 (E5O9G) Rabbit mAb (ChIP Formulated) #53575

Filter:
  • ChIP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZNF366 (E5O9G) Rabbit mAb (ChIP Formulated) recognizes endogenous levels of ZNF366 in ChIP analysis.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr57 of human ZNF366 protein.

    Background

    Dendritic cells (DCs) are the watchmen of the immune system, detecting a wide variety of pathogens and initiating robust immune responses. To respond to highly variable challenges, DCs have diversified into multiple phenotypical, anatomical, and functionally distinct cell types (1,2). Zinc finger protein 366 (ZNF366) was originally identified in a yeast two-hybrid screen for transcriptional regulators responding to estrogen (3,4). ZNF366 (dendritic cell (DC)-specific transcript, DC-SCRIPT) is a member of the Cys-Cys:His-His subfamily of zinc finger proteins (5). ZNF366 localizes to the nucleus and does not show transcriptional activation via transactivation assays but does interact with co-repressors (2,5). ZNF366 has been shown to be DC-specific and is expressed in myeloid DCs, plasmacytoid DCs, and Langerhans cells (5). ZNF366 knockout mice show an immune deficiency phenotype, with substantially decreased expression of the transcription factor IRF-8 (6). Loss of function in ZNF366 has been implicated in tumor cell evasion of immune responses (7). ZNF366 represents a novel DC marker that could be involved in transcriptional repression (1,5).

    Database Links

    UniProt ID: Q8N895


    Entrez-Gene Id: 167465


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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