ZO-3 (D57G7) XP® Rabbit mAb #3704
- WB
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Exogenous expression of the amino terminal portion of ZO-3 exerts a dominant negative effect that interferes with assembly of tight junctions and adherens junctions (9). However, additional evidence indicates that tight junctions do form in the absence of ZO-3 protein (10), and that mice lacking ZO-3 appear to develop normally (11).
- Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
- Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11.
- Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47.
- Umeda, K. et al. (2006) Cell 126, 741-54.
- Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66.
- Traweger, A. et al. (2003) J Biol Chem 278, 2692-700.
- Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36.
- Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24.
- Wittchen, E.S. et al. (2000) J Cell Biol 151, 825-36.
- Adachi, M. et al. (2006) Mol Cell Biol 26, 9003-15.
- Xu, J. et al. (2008) Mol Cell Biol 28, 1669-78.
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