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  1. Products /
  2. Proteomic Analysis Products /
  3. PTMScan® LysC Protease

PTMScan® LysC Protease #84748

    Product Information

    Product Usage Information

    For PTMScan® protocols, we recommend making a 5 mg/mL stock solution by resuspending 1 mg in 0.2 mL of 20 mM HEPES pH 8.0. Aliquot resuspended enzyme for single use. Store aliquots at -80°C. See the PTMScan® protocol for further details.

    Storage

    Lyophilized LysC has a shelf life of 12 months when stored at -20°C. Once reconstituted, the enzyme can be aliquoted and stored at -80°C for 6 months.

    Product Description

    Lysyl endopeptidase (LysC) hydrolyzes amide and peptide ester bonds on the carboxyl side of lysine residues and hydrolyzes S-aminoethylcysteine residues.

    Specificity / Sensitivity

    The activity of LysC is greater than or equal to 1 AU/mg.

    Source / Purification

    PTMScan® LysC Protease was produced recombinantly in E. coli, purified and provided lyophilized in 1-5% Tris and 20-40% mannitol. Purity was accessed by SDS-PAGE. One amidase unit (AU) of the enzyme produces 1 μmol of p-nitroaniline per min at pH 9.5 at 30°C.

    Background

    LysC cleaves proteins on the carboxyl side of lysine residues. This makes it a useful tool in protein sequencing and proteomic applications. The enzyme was originally purified from the Gram-negative soil bacteria Achromobacter lyticus (1). It has been found to work optimally between 30-37°C and pH ranges from 9.0-9.5 but will undergo degradation at 50°C and higher. Activity is maintained in 4 M urea or 0.1% SDS solutions for up to 6 hr at 30°C. Inhibitors of the enzyme include PMSF, DFP, and TLCK.

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PTMScan is a registered trademark of Cell Signaling Technology, Inc.
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