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  3. PTMScan® Nitro-Tyrosine Motif [Nitro-Y] Kit

PTMScan® Nitro-Tyrosine Motif [Nitro-Y] Kit #44462

PTMScan® Nitro-Tyrosine Motif [Nitro-Y] Kit: Image 1
Motif analysis using all Nitro-Tyrosine peptides enriched and identified by PTMScan® Nitro-Tyrosine Motif [Nitro-Y] Kit. Ten milligrams of HCT 116 human colon cancer cells were digested with trypsin and immunoprecipitated with PTMScan® Nitro-Tyrosine [Nitro-Y] Immunoaffinity Beads. Orbitrap Fusion Lumos mass spectrometer analysis identified a total of 711 unique sites. The motif logo shows that the Nitro-Y antibody is a general motif antibody that recognizes the Nitro-Y motif independent of protein context, without other amino acid preferences.
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To Purchase # 44462

Cat. # Size Qty. Price
44462S 1 Kit
10 assays
$2,502

Additional Information

This product is intended for peptide enrichment and mass spectrometry analysis. To learn more about our Proteomics Kits and Services please answer a few questions for our Proteomics group.

Contact the CST Proteomics Group

  • Product Includes
  • Related Products
Product IncludesVolume (with Count)
PTMScan® Nitro-Tyrosine [Nitro-Y] Immunoaffinity Beads #7394510 x 80 µl
PTMScan® IAP Buffer (10X) #999310 x 600 µl

Product Information

Storage

Antibody beads supplied in IAP buffer containing 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan™), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1).

Background

Nitric oxide (NO) is implicated in carcinogenesis (1), chronic infection, inflammation (2), and neurodegeneration (3). High levels of both superoxide and NO in tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5).

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
AcetylScan is a registered trademark of Cell Signaling Technology, Inc.
MethylScan is a registered trademark of Cell Signaling Technology, Inc.
PhosphoScan is a registered trademark of Cell Signaling Technology, Inc.
UbiScan is a registered trademark of Cell Signaling Technology, Inc.
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