PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Kit #12170
Additional Information
This product is intended for peptide enrichment and mass spectrometry analysis. To learn more about our Proteomics Kits and Services please answer a few questions for our Proteomics group.
Product Information
Product Usage Information
Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit.
Storage
Protocol
Product Description
Background
- Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
- Pinna, L.A. and Allende, J.E. (2009) Cell Mol Life Sci 66, 1795-9.
- St-Denis, N.A. and Litchfield, D.W. (2009) Cell Mol Life Sci 66, 1817-29.
- Trembley, J.H. et al. (2009) Cell Mol Life Sci 66, 1858-67.
- Perez, D.I. et al. (2011) Med Res Rev 31, 924-54.
- Dominguez, I. et al. (2009) Cell Mol Life Sci 66, 1850-7.
- Meggio, F. and Pinna, L.A. (2003) FASEB J 17, 349-68.
Limited Uses
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