PTMScan® Pilot Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit #14478
Additional Information
This product is intended for peptide enrichment and mass spectrometry analysis. To learn more about our Proteomics Kits and Services please answer a few questions for our Proteomics group.
Product Information
Product Usage Information
Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit.
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Product Description
Background
Product Citations: 32
- Lundby, A. et al. (2019) Cell 179, 543-560.e26.
- Weber, A. et al. (2019) J Virol 93.
- Thompson, S.M. et al. (2018) Int J Hyperthermia 34, 812-823.
- Goel, R.K. et al. (2018) Mol Cell Proteomics 17, 925-947.
- Jeitany, M. et al. (2018) EMBO Mol Med 10, .
- Venkatesh, H.S. et al. (2017) Nature 549, 533-537.
- Patterson et al. (2016) Proc Natl Acad Sci U S A 113, E2094.
- Gu, H. et al. (2016) Mol Cell Proteomics 15, 692-702.
- Standish, A.J. et al. (2016) J Mol Biol 428, 4197-4208.
- Palacios-Moreno, J. et al. (2015) PLoS Comput Biol 11, e1004130.
- Stokes, M.P. et al. (2015) Proteomes 3, 160-183.
- van der Mijn, J.C. et al. (2015) J Proteomics 127, 259-63.
- Verma, R. et al. (2014) J Exp Med 211, 1715-22.
- Paardekooper Overman, J. et al. (2014) Mol Cell Biol 34, 2874-89.
- Yoshida, T. et al. (2014) Clin Cancer Res 20, 4059-4074.
- Gu, T.L. et al. (2011) PLoS One 6, e15640.
- Pighi, C. et al. (2011) Cell Oncol (Dordr) 34, 141-53.
- Brave, S.R. et al. (2011) Mol Cancer Ther 10, 861-73.
- Andersen, J.N. et al. (2010) Sci Transl Med 2, 43ra55.
- Knowlton, M.L. et al. (2010) PLoS One 5, e13587.
- Guo, A. et al. (2008) Proc Natl Acad Sci U S A 105, 692-7.
- Rikova, K. et al. (2007) Cell 131, 1190-203.
- Gu, T.L. et al. (2007) Leukemia 21, 563-6.
- Gu, T.L. et al. (2007) Blood 110, 323-33.
- Mercher, T. et al. (2006) Blood 108, 2770-9.
- Griswold, I.J. et al. (2006) Mol Cell Biol 26, 6082-93.
- Gu, T.L. et al. (2006) Blood 108, 4202-4.
- Goss, V.L. et al. (2006) Blood 107, 4888-97.
- Zhang, Y. et al. (2005) Mol Cell Proteomics 4, 1240-50.
- Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
- Chua, X.Y. et al. (2020) Mol Cell Proteomics , .
- van Alphen, C. et al. (2020) Mol Cell Proteomics , .
Limited Uses
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