PTMScan® Wild Type Alpha-Lytic Protease (WaLP) #33036
Product Information
Product Usage Information
PTMScan® Wild Type Alpha-Lytic Protease (WaLP) is provided for use with Cell Signaling Technology's patented PTMScan® protocol in the initial protein digestion step. Samples in urea lysis buffer need to be diluted to 2M or less urea prior to addition of WaLP. Dilute the 0.4 mg/ml stock 1:100 for final digest and incubate overnight at 37°C. Aliquot and store at -20ºC. Consult the specific PTMScan® kit and protocol for more details on the appropriate protease before digesting any protein samples.
Storage
Product Description
Specificity / Sensitivity
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
- Whitaker, DR. The alpha-Lytic Protease. Methods in Enzymology, 19, 599-613
- Sohl, JL, et al., , IntramolecularChaperones and Protein Folding, Landes Company, Austin TX (1995) ISBN 978-1570592928 pp. 61-83
- Bauer, C.A. et al. (1981) Eur J Biochem 120, 289-94.
- Bachovchin, W.W. et al. (1981) Proc Natl Acad Sci U S A 78, 7323-6.
- Olson, M.O. et al. (1970) Nature 228, 438-42.
- Meyer, J.G. et al. (2014) Mol Cell Proteomics 13, 823-35.
Limited Uses
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