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Anti-biotin, HRP-linked Antibody

Anti-biotin, HRP-linked Antibody #7075

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Supporting Data

REACTIVITY	All
SENSITIVITY
MW (kDa)
Source/Isotype	Goat

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Description

Affinity purified goat anti-biotin antibody is conjugated to horseradish peroxidase. This product has been optimized to detect biotinylated protein markers.

Product Usage Information

For western blots, incubate membrane and diluted antibody in 5% w/v nonfat dry milk, 1x TBS, 0.1% Tween(R) 20 at room temperature for one hour prior to detection. Note: Sodium azide is an inhibitor of HRP-binding and should not be included in the dilution buffer.

Recommended Antibody Dilutions:

1:1000-1:3000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. This product is stable for 60 months when stored at -20C.

Protocol

Specificity / Sensitivity

This antibody may cross react with endogenously expressed biotinylated proteins. Careful titration may be needed.

Species Reactivity:

All Species Expected

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

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