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Anti-Human IgM, Fc5µ Fragment Specific, HRP-Linked Antibody

Anti-Human IgM, Fc5µ Fragment Specific, HRP-Linked Antibody #51821

Supporting Data

REACTIVITY	H
SENSITIVITY
MW (kDa)
Source/Isotype	Goat

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

Affinity purified goat Anti-Human IgM, Fc5µ Fragment Specific antibody is conjugated to horseradish peroxidase (HRP). This product has been optimized for use as a secondary antibody in Western blotting and ELISA applications.

Product Usage Information

Reconstitute with 1.5 ml of dH₂0 to create a 0.8 mg/ml working solution. Centrifuge if the solution is not clear. It is recommended to prepare the working solution on day of use.

Recommended Antibody Dilutions:

ELISA (chromogenic substrates): 1:5K-1:100K

Western Blotting (chromogenic substrates): 1:5K - 1:100K

Western Blotting (ECL substrates): 1:10K - 1:200K

Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.

Storage

Supplied lyophilized with a formulation of 0.01M Sodium Phosphate (pH 7.6), 0.25M NaCl, and 15 mg/ml bovine serum albumin (BSA). Store lyophilized at 4ºC. In lyophilized form, the product is stable for 24 months. Once in solution, store at -80ºC or add glycerol (ACS grade or better) to a final concentration of 50% and store at -20ºC. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles.

Specificity / Sensitivity

Anti-Human IgM, Fc5µ Fragment Specific, HRP-Linked Antibody detects the Fc5µ portion of human IgM heavy chain but does not react with human IgG or IgA, or the light chains of human immunoglobulins. This antibody has minimal cross-reaction with bovine serum proteins. It may cross-react with IgM from other species.

Species Reactivity:

Human

Source / Purification

Anti-Human IgM, Fc5µ Fragment Specific, HRP-Linked Antibody is produced by immunizing goats with human IgM. The Anti-Human IgM, Fc5µ Fragment is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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